Identifying the arcuate fasciculus in DTIStudio using a multiple ROI approach

Written 8/8/08 by Aimee Zisner. Updated 02/17/2009 by Owen Phillips. Email Dr. Katherine Narr if you have any questions. This protocol explains how to identify the arcuate fasciculus (AF) in DTIStudio. For more information on identifying white matter tracts using DTIStudio, see


Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.


To identify the AF:

First ROI: Identify the most inferior axial slice in which the fornix is visible. In the color map, the fornix is a blue circular structure (marked by the arrow; 1). Identify the coronal slice in which the indicated green fibers are most intense (2). Select all green fibers in this region (3).
Locating the first ROI
First ROI
First ROI
Second ROI Second ROI: Using the axial slice identified for the first ROI, select the bundle of fibers projecting laterally to the sagittal stratum (SS). * Be sure to remove all fibers that are clearly not a part of the tract.
Sagittal view of AF Sagittal view of the arcuate fasciculus.


  1. Wakana S, Caprihan A, Panzenboeck MM, Fallon JH, Perry M, Gollub RL, Hua K, Zhang J, Jiang H, Dubey P, Blitz A, van Zijl P, Mori S (2007). Reproducibility of quantitative tractography methods applied to cerebral white matter. Neuroimage. 2007 Jul 1;36(3):630-44. Click here to view.

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