Identifying the inferior fronto-occipital fasciculus in DTIStudio using a multiple ROI approach

Written 7/25/08 by Aimee Zisner. Updated 02/17/2009 by Owen Phillips. Email Dr. Katherine Narr if you have any questions. This protocol explains how to identify the inferior fronto-occipital fasciculus (IFO) in DTIStudio. For more information on identifying white matter tracts using DTIStudio, see

Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.

To identify the IFO:

First ROI: Identify the coronal slice in which the green fibers connecting the temporal and parietal lobes are most intense (marked by arrows; 1). This slice is typically a few slices anterior to the splenium of the corpus collosum. Select these fibers (2).
Locating the first ROI
First ROI
Second ROI Second ROI: Identify the coronal slice in which the tract bunches, typically at the anterior portion of the fornix. Select the circular cluster of fibers running through the corticospinal tract.
* Be sure to remove all fibers that are clearly not a part of the tract. Note the inferior longitudinal fasciculus, which has fibers beginning in the temporal lobe and projects similarly to the IFO.
Sagittal view of IFO Sagittal view, left.
Ventral axial view, right.
Ventral view of IFO


  1. Wakana S, Caprihan A, Panzenboeck MM, Fallon JH, Perry M, Gollub RL, Hua K, Zhang J, Jiang H, Dubey P, Blitz A, van Zijl P, Mori S (2007). Reproducibility of quantitative tractography methods applied to cerebral white matter. Neuroimage. 2007 Jul 1;36(3):630-44. Click here to view.


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