Identifying the inferior longitudinal fasciculus in DTIStudio using a multiple ROI approach

Written 8/8/08 by Aimee Zisner. Updated 02/17/2009 by Owen Phillips. Email Dr. Katherine Narr if you have any questions. This protocol explains how to identify the inferior longitudinal fasciculus (ILF) in DTIStudio. For more information on identifying white matter tracts using DTIStudio, see

Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.

To identify the ILF:

First ROI First ROI: Identify the coronal slice that crosses the posterior edge of the cingulum. Select all fibers included in the hemisphere of interest.
Second ROI: Identify the most posterior coronal slice in which the frontal lobe is disconnected from the temporal lobe. Select all fibers in the temporal lobe.

* Be sure to remove all fibers that are clearly not a part of the tract.
Second ROI
Sagittal view of the inferior longitudinal fasciculus Sagittal view of the inferior longitudinal fasciculus


  1. Wakana S, Caprihan A, Panzenboeck MM, Fallon JH, Perry M, Gollub RL, Hua K, Zhang J, Jiang H, Dubey P, Blitz A, van Zijl P, Mori S (2007). Reproducibility of quantitative tractography methods applied to cerebral white matter. Neuroimage. 2007 Jul 1;36(3):630-44. Epub 2007 Mar 20. Click here to view.


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