Identifying the uncinate fasciculus in DTIStudio using a multiple ROI approach

Written 8/8/08 by Aimee Zisner. Updated 02/17/2009 by Owen Phillips. Email Dr. Katherine Narr if you have any questions. This protocol explains how to identify the uncinate fasciculus (UF) in DTIStudio. For more information on identifying white matter tracts using DTIStudio, see


Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.


To identify the UF:

First ROI: Identify the most posterior coronal slice in which the green fibers connecting the temporal and frontal lobes separate (marked by the arrow; 1). This slice is typically located at the anterior portion of the fornix in the midsagittal view. Select all fibers in the temporal lobe (2).
Locating the first ROI
First ROI
Second ROI Second ROI: In the same coronal slice, select the fibers projecting to the frontal lobe. If these fibers are not clearly delineated, select a slice slightly more anterior when they become visible.
* Be sure to remove all fibers that are clearly not a part of the tract.
Sagittal view of UF Sagittal view of the uncinate fasciculus.


  1. Wakana S, Caprihan A, Panzenboeck MM, Fallon JH, Perry M, Gollub RL, Hua K, Zhang J, Jiang H, Dubey P, Blitz A, van Zijl P, Mori S (2007). Reproducibility of quantitative tractography methods applied to cerebral white matter. Neuroimage. 2007 Jul 1;36(3):630-44. Click here to view.


Related Protocols